Pengaruh periode pra-kondisi dan penutupan sungkup terhadap daya hidup planlet karet (Hevea brasiliensis Muell. Arg) Effect of pre-condition period and vessel closure on the survival rate of rubber (Hevea brasiliensis Muell. Arg) plantlets


  • Masna Maya SINTA



Pre-condition, in vitro, rootstock, vessel closure, acclimatization


Acclimatization of plantlets is a critical stage in the micropropagation of many plants. An experiment was conducted to determine the effect of pre-condition period and vessel closure on the growth and survival rate of rubber (Hevea brasiliensis Muell.Arg.) plantlets derived from in vitro microcutting during acclimatization. Plantlets were planted in plastic pots containing mixed growing media after being conditioned in ex vitro environment for 0, 3 and 6 days. Five closure vessel treatments were closed pots placed in opened container, opened pots in closed glass container, closed pots in closed glass container, opened pots in closed plastic container, and closed pots in closed plastic container. Observation on leaf conditions, rooting frequency, and plant height were conducted at 1.5 months and on the percentage of survive plantlets at 1.5 and 3 months after acclimatization. The results showed that pre-condition was required to increase survival rate and growth of the plantlets. Pre-condition period of six days gave a higher survival rate than 0 and 3 days which reached 100% and 93% on opened pot in closed plastic container and closed pot in opened container, respectively after 1.5 months and was reduced to 80% after three months of acclimatization. The highest formation of new leaves and roots were also obtained on six days pre-condition period. Plantlets with pre-condition for six days and were planted on closed pots in an opened container had the best rooting frequency which was 90%. The result showed that the highest survival rate (80%) of rubber plantlets after three months was obtained when the plantlets were pre-conditioned in ex vitro conditions for six days before acclimatization and planted on opened pots in a closed plastic container or closed pots in an opened container.


Download data is not yet available.


Anonim (2006). Bagan Warna Daun Diunduh dari: Juknis BWD.pdf, [ 22 September 2011].

Carron MP, L Lardet, A Leconte, C Boko, BG Dea & J Keli (2003). Field growth and rubber yield of Hevea brasiliensis (Muell. Arg.) from budded versus in vitro micropropagated plants from clone IRCA 18. In: Economou & Read (eds.). Proc the First Internat. Symp on Acclim & Establish of Micropropagated Plants p. 283-293.

Carron MP, Y Le Roux, J Tison, BG Dea, V Caussanel, J Clair & J Keli (2000). Compared root system architectures in seedlings and in vitro plantlets of Hevea brasiliensis, in the initial years of growth in the field. Plant & Soil 223(2), 73-85.

Chen U, CH Hsia, DC Agrawall & HS Tsay (2006). Influence of ventilation closures on plant growth parameters, acclimation and anatomy of leaf surface in Scrophularia yoshimurae Yamazaki - a medicinal plant native to Taiwan. Bot Studies 47, 259-266.

Dibi K, C Boko, S Obouayeba, M Gnagne, GB Dea, MP Carron & AP Anno (2010). Field growth and rubber yield of in vitro micropropagated plants of clones PR 107, IRCA 18 and RRIM 600 of Hevea brasiliensis (Muëll. Arg.). Agric Biol J North America 1(6), 1291-1298.

Ebrahim N, R Shibli, I Makhadameh, M Shatnawi & A Abu-Ein (2007). In vitro propagation and in vivo acclimatization of three coffee cultivars (Coffea arabica L.) from Yemen. J World Appl Sci 2(2), 142-150.

Hazarika BN (2003). Acclimatization of tissue-cultured plants. Current Sci 85(12), 1704-1712.

Kadlecek P, I Ticha, D Haisel, V Capkova & C Schafer (2001). Importance of in vitro pretreatment for ex vitro acclimatization and growth. Plant Sci 161, 695- 701.

Mishara Y, P Patel & SA Ansari (2011). Acclimatization and micropropagation of micropropagated plants of Bambusa tulda Roxb. Asian J Exp Biol Sci 2(3), 498- 501.

Nizam K & S Te-Chato (2009). Optimizing of root induction in oil palm plantlets for acclimatization by some potent plant growth regulators (PGRs). J Agric Technol 5(2), 371-383.

Nuraini (2004). Upaya aklimatisasi planlet krisan (Chrisanthemum morifolium): Pengaruh prakondisi, media tanam dan jenis naungan. Tesis, Palembang, Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Sriwijaya.

Nurhaimi-Haris & MP Carron (2007). Toward the development of rootstock clones for high productivity in rubber plants (Hevea brasiliensis Muell.Arg.). Progress Report. Indonesian Biotechnol Res Inst for Estate Crops

Nurhaimi-Haris, Sumaryono, PD Kasi, Siswanto, Sumarmadji & MP Carron (2008). Achievements in the implementation of microcutting technology for clonal rubber rootstock propagation. In : Proc Internat Workshop Rubber Planting Materials. Bogor, 28-29 October 2008, p.117-128.

Nurhaimi-Harris, NS Ayuningtyas & JH Suparto (2011). Pengaruh ventilasi terhadap morfologi, stomata dan kadar klorofil tunas karet yang diperbanyak melalui microcutting. Menara Perkebunan 79(2), 58-64.

Pospisilova J, H Synkova, D Haisel & S Semoradova (2007). Acclimation of plantlets to ex vitro conditions: Effects of air humidity, irradiance, CO2concentration and abscisic acid (a review). Acta Hort 748, 29-38.

Sumaryono, PD Kasi, Nurhaimi-Haris & MP Carron (2008). Aklimatisasi planlet karet yang diperbanyak melalui teknologi microcutting. Dalam : Pros Lokakarya Nasional Agribisnis Karet, Yogyakarta, 20-21 Agustus 2008, p.323-329.

Sumaryono, MM Sinta & Nurhaimi-Haris (2012). Daya hidup planlet karet asal in vitro microcutting pada berbagai periode penutupan sungkup plastik dan komposisi media tumbuh. Menara Perkebunan 80(2), 25-31.

Zobayed SMA (2005). Ventilation in micropopagation. In: T. Kozai et al. (eds.) Photoautotropic (Sugar- Free Medium) Micopopagation as A New Micro- propagation and Transplant Production System. The Netherlands, Springer. p.147-186.








How to Cite

SINTA, M. M., NURHAIMI-HARIS, ., & SUMARYONO, . (2016). Pengaruh periode pra-kondisi dan penutupan sungkup terhadap daya hidup planlet karet (Hevea brasiliensis Muell. Arg) Effect of pre-condition period and vessel closure on the survival rate of rubber (Hevea brasiliensis Muell. Arg) plantlets. Menara Perkebunan, 81(1).




Most read articles by the same author(s)

1 2 3 4 5 > >>