Biocontrol activity of endophytic bacteria from cocoa against Phytophthora sp. and Colletotrichum sp.
DOI:
https://doi.org/10.22302/iribb.jur.mp.v91i1.522Abstract
Cocoa (Theobroma cacao L.) and chili pepper (Capsicum annuum L.) are commonly cultivated by farmers, and in some cases, both crops are grown together in intercropping systems. The main pathogen infected cocoa is the Phytophthora sp. fungus, which causes fruit rot, stem cancer, and leaf blight, while Colletotrichum sp. causes anthracnose in chili pepper plants. The ability of endophytic bacteria to inhibit the growth of fungi and bacterial pathogens is well known. In this study, the biocontrol ability of endophytic bacteria against fungal pathogens was tested, and a biocontrol preliminary test was also observed by examining their potential inhibition on Chromobacterium violaceum called antiquorum sensing test. Endophytic bacteria were isolated from cocoa plants, while Phytophthora and Colletotrichum were isolated from cocoa and chili pepper plants that showed symptoms of fruit rot and anthracnose. A total of 34 endophytic bacterial isolates were successfully obtained, with 10 isolates from leaves (DK), 12 isolates from branches (RK), and 12 isolates from roots (KA) of cocoa plant ICCRI 4. Sixteen isolates showed quorum sensing ability, which AHL degradation index ranging from 0.44 – 1.56. Antagonistic tests showed that 11 out of 16 isolates had strong antibiosis against Phytophthora sp., with inhibition zones ranging from 0.6-1.35 cm. Meanwhile, 10 out of 16 isolates had strong antibiotics against Colletotrichum sp., with clear zones ranging from 0.6 – 1.1 cm. The three best endophytic bacterial isolates that had a combination of antiquorum sensing ability on C. violaceum and biocontrol against Phytophthora sp. and Colletotrichum sp. were RK 11, KA 1, and KA 8.
[Keywords: antagonist, ahl lactonase, plant beneficial microorganism]
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Abidin, Z., Aini, L. Q., & Abadi, A. L. (2015). Pengaruh bakteri Bacillus sp. dan Pseudomonas sp. terhadap pertumbuhan jamur patogen Sclerotium rolfsii Sacc. penyebab penyakit rebah semai pada tanaman kedelai. Jurnal HPT, 3(1), 1-10. http://repository.ub.ac.id/ id/eprint/129928
Agisha, V. N., Kumar, A., Eapen, S. J., Sheoran, N., & Suseelabhai, R. (2019). Broad-spectrum antimicrobial activity of volatile organic compounds from endophytic Pseudomonas putida BP25 against diverse plant pathogens. Biocontrol Science and Technology, 29(11), 1069-1089. https://doi.org/10.1080/09583157. 2019.1657067
Alexopoulos, C. J., Mims, C. W., & Blackwell, M. (1996). Introductory mycology. John Wiley & Sons. Singapore. p. 244 - 324.
Alsultan, W., Vadamalai, G., Khairulmazmi, A., Saud, H. M., Al-Sadi, A. M., Rashed, O., A. K. M. J., & Nasehi, A. (2019). Isolation, identification and characterization of endophytic bacteria antagonistic to Phytophthora palmivora causing black pod of cocoa in Malaysia. European Journal of Plant Pathology, 155, 1077-1091. https://doi.org/10.1007/s10658-019-01834-8
Anggraeni, W., Elvi, R. P., Wardoyo, & Rahmawati. (2019). Isolation and identification of fungi in cayenne pepper fruit (Capsicum frutescens L.) The symptomatic anthracnose from farmland In orange hamlet. Jurnal Protobiont. 8(2), 94 – 100. http://dx.doi.org/10.26418/protobiont.v8i2. 34058
Arneti, Y. L., & Edriwilya, R. (2020). Effectiveness of papaya leaf extract in vitro against Colletotrichum gloeosporioides causing anthracnose disease in chili plants. Jurnal Proteksi Tanaman (Journal of Plant Protection). 4(1), 1 – 10. https://doi.org/10.25077/jpt.4.1.1-10.2020
Aryani, P., Kusdiyantini, E., & Suprihadi, A. (2020). Isolation of endophytic bacteria of reed leaves (Imperata cylindrica) and its secondary metabolites that have the potential to be antibacterial. Jurnal Akademika Biologi, 9(2), 20-28. https://doi.org/ 10.1186/s12906-020-03095-z
Bacon, C. W., Hinton, D. M., & Mitchell, T. R. (2017). Is quorum signaling by mycotoxins a new risk-mitigating strategy for bacterial biocontrol of Fusarium verticillioides and other endophytic fungal species?. Journal of Agricultural and Food Chemistry, 65(33), 7071-7080. https://doi.org/10.1021/acs.jafc.6b03861
Barnett, H. L., & Hunter, B. B. (1972). Illustrated genera of imperf ect fungi. Third Edition. Burgess Publishing. Company.
De França Bettencourt, G. M., Degenhardt J., Dos Santos G. D., Vicente V. A., & Soccol C. R. (2021). Metagenomic analyses, isolation and characterization of endophytic bacteria associated with Eucalyptus urophylla BRS07-01 in vitro plants. World Journal of Microbiology and Biotechnology, 37, 1-16. https://doi.org/10.1007/s11274-021-03127-x
Camila, F. S., Temaja, I. G. R., & Khalimi, K. (2019). Potensi bakteri endofit dalam menekan pertumbuhan Phytophthora sp. (Butler) Secara In Vitro. Jurnal Agroekoteknologi Tropika, 8(4), 388-398.
Djaenuddin, & Nurasiah. (2016). Interaksi bakteri antagonis dengan tanaman: Ketahanan terinduksi pada tanaman jagung. Iptek Tanaman Pangan, 11(2), 2016.
Eris, D. D., Munif, A., Soekarno, B. P. W., & Purwantara, A.. (2017). Screening and potential of endophytic bacteria of arecaceae plant origin as biological control agents of the fungus Pestalotiopsis sp. causes of leaf spot disease in coconut kopyor (Cocos nucifera). Menara Perkebunan, 85(1),19-27. https://doi.org/ 10.22302/iribb.jur.mp.v85i1.235
Harahap, Hakim, T. F., Lubis, L., & Hasanuddin. (2013). Efek temperatur terhadap virulensi jamur Colletotrichum sp. Penz. Sacc. penyebab penyakit antraknosa pada tanaman kakao (Theobroma cacao L.). Jurnal Online Agroekoteknologi, 2(1), 411-420. https://doi.org/10.32734/jaet.v2i1.5842
Hidayatun, N., Susilowati, D. N., & Mulya, K. (2011). Identification of 26 isolates of endophytic bacteria and rice filospheres with sequencing analysis16s RDNA. Berita Biologi, 10 (4). https://doi.org/10.14203/beritabiologi. v10i2.1978
Ibrahim, R., Hidayat, S. H, & Widodo. (2017). Morphological diversity, genetics, and pathogenicity of Colletotrichum acutatum causes chili anthracnose in Java and Sumatra. Jurnal Fitopatologi Indonesia,13(1), 9-16. https://doi.org/10.14692/jfi.13.1.9
Indrawangsa, G. D., Sudarma, I. M., & Singarsa, I. D. P. (2017). Endophytic fungus inhibitory test against Phytophthora sp. (Butler) butler causes cocoa fruit rot disease in vitro. E-Jurnal Agroekoteknologi Tropika, 6 (3), 229-238. https://doi.org/10.13057/biodiv/d220519
ISC Indonesian Statistic Center. (2022). Produksi tanaman perkebunan 2019-2021. Pejabat Pengelola Informasi dan Dokumentasi Badan Pusat Statistik [ID].
Joo, H. S., Deyrup, S. T., & Shim, S. H. (2021). Endophyte-produced antimicrobials: a review of potential lead compounds with a focus on quorum-sensing disruptors. Phytochemistry Reviews, 20, 543-568. https://doi.org/10.1007/ s11101-020-09711-7
Lacava, P. T., Bogas, A. C., & Cruz, F. D. P. N. (2022). Plant growth promotion and biocontrol by endophytic and rhizospheric microorganisms from the tropics: A review and perspectives. Frontiers in Sustainable Food Systems, 6, 796113. https://doi.org/10.3389/fsufs.2022. 796113
Muzuni, H. & Yanti, N. A. (2020). Morphological characterization of Phytophthora sp. Origin of cocoa fruit Olo-oloho village, Konawe county, Southeast Sulawesi. Jurnal Penelitian Biologi /Journal of Biological Research, 7 (1), 1064-1069. http://dx.doi.org/10.33772/biowallacea. v7i1.11812
Murtado, A., Mubarik, N. R., & Tjahjoleksono A. (2020). Isolation and characterization endophytic bacteria as biological control of fungus Colletotrichum sp. on onion plants (Allium cepa L.). In IOP Conference Series: Earth and Environmental Science, 457 (1), 012043. https://doi.org/10.1088/17551315/ 457/1/012043
Nasamsir. (2014). Respons pertumbuhan bibit kakao (Theobroma cacao L.) terhadap aplikasi pupuk organik cair pada jenis aksesi buah kakao yang berbeda. Jurnal Ilmiah Universitas Batanghari Jambi, 14(3), 91-100. http://dx.doi.org/10. 33087/jiubj.v14i3.264
Najah, Naelun, L., Suhartanto, N. M., & Widodo. (2016). Control of Colletotrichum spp. carried away chili seeds with microwave exposure. Jurnal Fitopatologi Indonesia, 12(4),115-123.
Novita, H., Rusmana, I., Yuhana, M., Pasaribu, F. H., & Lusiastuti, A. M. (2016). In vitro test of bacteria anti quorum sensing degradation acyl homoserine lactone Aeromonas hydrophila. Jurnal Riset Akuakultur, 11(3), 291-296. https://doi.org/10.15578/jra.11.3.2016.291-296
Nurfianti, U. (2019). Pengamatan gejala infeksi Phytophthora palmivora penyebab penyakit busuk buah pada kakao. Biocelebes, 13(3), 253-261.
Ouattara, A., Coulibaly, K., Konate, I., Ismaë, B., Tidou, A. S., & Filali-Maltouf, A. (2019). Selection of cocoa tree (Theobroma cacao Linn) endophytic bacteria solubilizing tri-calcium phosphate, isolated from seedlings grown on soils of six producing regions of Côte d’Ivoire. Advances in Microbiology, 9(9), 842-852. https://doi.org/10.4236/aim.2019.99051
Perrine F., & Walker. (2020). Review Phytophthora sp. cocoa interaction. Journal of Fungi, 6, 167. DOI: https://doi.org/10.3390/jof6030167
Prihatiningsih, N., Djatmiko, H. A., & Lestari, P. (2022). Antagonistic feature displayed by endophytic bacteria consortium for control rice pathogens. Jurnal Hama dan Penyakit Tumbuhan Tropika, 22(2), 154-161. https://doi.org/10.23960/jhptt.222154-161
Puri, A., Padda, K. P., & Chanway, C. P. (2020). Can naturally-occurring endophytic nitrogen-fixing bacteria of hybrid white spruce sustain boreal forest tree growth on extremely nutrient-poor soils?. Soil Biology and Biochemistry, 140, 107642. https://doi.org/10.1016/j.soilbio.2019. 107642
Purwanto, Safira, U. M., Pasaribu, F. H., & Bintang, M.(2014). Isolasi bakteri endofit dari tanaman sirih hijau (Piper betle L.) dan potensinya sebagai penghasil senyawa antibakteri. Current Biochemistry, 1(1), 51-57.
Rubiyo. (2013). Inovasi teknologi perbaikan bahan tanam kakao di Indonesia. Buletin RISTRI, 4(3), 199-214. https://doi.org/10.21082/jtidp.v4n3. 2013.p199-214
Shan, W., Zhou, Y., Liu, H., & Yu, X. (2018). Endophytic actinomycetes from tea plants (Camellia sinensis): isolation, abundance, antimicrobial, and plant-growth-promoting activities. BioMed Research International, 2018, 1-12. https://doi.org/10.1155/2018/1470305
Sharma, M., & Mallubhotla, S. (2022). Diversity, antimicrobial activity, and antibiotic susceptibility pattern of endophytic bacteria sourced from Cordia dichotoma L. Frontiers in Microbiology, 13. https://doi.org/ 10.3389/fmicb .2022.879386
Sharma, S., Duveiller, E., Khetarpal, R. K., & Sharma, S. M. (2018). Alternatives to chemical fungicides for the control of fusarium wilt of tomato: challenges and opportunities. Plant Pathology, 67(6), 1281-1293.
Sudirga, S. K. (2016). Isolation and Identification of the fungus Colletotrichum spp. PCS isolates causing anthracnose disease in large chili fruits (Capsicum annuum L.) in Bali. Jurnal Metamorfosa. 3(1), 23-30.
Surujdeo-Maharaj, S., Sreenivasan, T. N., Motilal, L. A., & Umaharan, P. (2016). Black pod and other phytophthora induced diseases of cacao: History, biology, and control. Cacao diseases: A history of old enemies and new encounters, pp.213-266. https://doi.org/10.1007/978-3-319-24789-2_7
Suryanti, E. (2019). The potential of AHL lactonase-producing bacteria as a control of potato soft rot by Dickeya dadantii. Thesis. Faculty of Mathematics and Natural Sciences. Institut Pertanian Bogor (IPB).
Vasavi, H. S., Ananthapadmanabha, B. A., & Punchapady, D. R. (2014). Anti-quorum sensing activity of Psidium guajava L. flavonoids against Chromobacterium violaceum and Pseudomonas aeruginosa PAO1. Microbiology and Immunology, 58, 286–29. https://doi.org/10. 1111/1348-0421.12150
Wartono & Efi, T. (2021). Cocoa fruit rot disease pathogens: Character and pathogenicity of Phytophthora Sp. isolates of Pakuwon Origin, Sukabumi. Jurnal Tanaman Industri dan Penyegar, 8(1), 49 – 58. https://doi.org/10.21082/jtidp.v8n1.2021.p49-58
Widiantini, F., Endah, Y., & Kurniawan, A. (2020). Penghambatan pertumbuhan Rhizoctonia oryzae dan Cercospora oryzae oleh senyawa volatil yang dihasilkan bakteri endofit padi. Jurnal Agrikultura, 31(1), 61-67. https://doi.org/10. 24198/agrikultura.v31i1.27323
Widiyatmoko, Eko, W., Christiani, Y., Indrabayu, & Yoga A. H. (2019). Efektivitas antagonisme Trichoderma virens terhadap fitopatogen Phytophthora sp. pada tanaman kakao (Theobroma cacao L.). Jurnal Pertanian Tropik, 6(1), 101–107. https://doi.org/10.32734/jpt. v6i1.3046
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