The effect of chitin on the effectiveness of Beauveria bassiana formulation product to control cocoa pod borer in vitro


  • Eka Nurhangga National Research and Innovation Agency
  • Winda Nawfetrias National Research and Innovation Agency
  • Nailulkamal Djamas National Research and Innovation Agency
  • Akhmad Jufri National Research and Innovation Agency



bioinsecticide, chitin, entomopathogen, fungus, virulence


Fungal entomopathogens are suggested to be used as a bioinsecticide due to their biological persistence and ecological friendliness. Beauveria bassiana is an entomopathogenic fungus that can effectively control insect pests, including cocoa pod borer. This study aims to determine the level of effectivity of the B. bassiana (Bb) formulation product from Palu against cocoa pod borer pupae in vitro . This research used a completely randomized design (CRD) consisting of two factors: conidia concentrations and chitin treatments, which were repeated thrice. The conidia concentrations consisted of two levels, that were 106 and 107 conidia/mL. The chitin treatment included seven treatments: isolate from Kediri without formulation with chitin (Bb-K), isolate from Palu without formulation with chitin (Bb-P0), four formulations of isolates from Palu with the addition of chitin to various growth media (Bb-P1, Bb-P2, Bb-P3, and Bb-P4), and control (without Bb suspension). Bb-P0 and Bb-P2 treatments at a concentration of 107conidia/mL began to colonize CPB on the third day after the treatment application, while the other formulations started on the fifth day. However, Bb-P0 showed the lowest infection rate at the end of the observation. Contrarily, the data on the pupa-infected ratio showed that the Bb-P2 treatment was the highest compared to other treatments. It conformed to the adult emergence ratio that Bb-P2 exhibited the lowest, which means Bb-P2 has the most virulence of other formula. The result showed that PDA and PDB media-supplemented chitin was the most effective for culturing B. bassiana origin Palu before mass production.


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Afandhi, A., Choliq, F. A., Fernando, I., Marpaung, Y. M. A. N., & Setiawan, Y. (2022). Occurrence of soil-inhabiting entomopathogenic fungi within a conventional and organic farm and their virulence against Spodoptera litura. Biodiversitas Journal of Biological Diversity, 23(2), Article 2. biodiv/d230263

Afandhi, A., Rachmawati, R., Syib’li, M. A., & Zain, H. A. U. (2023). Performance and virulence of the entomopathogenic fungi Beauveria bassiana grown in media derived from biodegradable agricultural wastes enriched with cricket powder. AGRIVITA, Journal of Agricultural Science, 45(2), Article 2.

Bagariang, W., Kurniati, A., Lestrari, T. M. P., Mahmudah, D., Suyanto, H., & Cahyana, N. A. (2023). Uji media padat Beauveria Bassiana terhadap mortalitas, pembentukan pupa dan kemunculan imago Spodoptera litura Fabr. Agro Wiralodra, 6(1), 1–8. /agrowiralodra.v6i1.86

Bamisile, B. S., Akutse, K. S., Siddiqui, J. A., & Xu, Y. (2021). Model application of entomopathogenic fungi as alternatives to chemical pesticides: Prospects, challenges, and insights for next-generation sustainable agriculture. Frontiers in Plant Science, 12.

Barreto, C. C., Staats, C. C., Schrank, A., & Vainstein, M. H. (2004). Distribution of chitinases in the entomopathogen Metarhizium anisopliae and effect of N-acetylglucosamine in protein secretion. Current Microbiology, 48(2), 102–107.

Bihal, R., Al-Khayri, J. M., Banu, A. N., Kudesia, N., Ahmed, F. K., Sarkar, R., Arora, A., & Abd-Elsalam, K. A. (2023). Entomopathogenic fungi: An eco-friendly synthesis of sustainable nanoparticles and their nanopesticide properties. Microorganisms, 11(6), 1617. 10.3390/microorganisms11061617

Deb, L., Rajesh, T., & Hajong, M. (2017). Growth of Beauveria bassiana in different solid media. Trends in Biosciences, 10(23), 4815–4817

Doolotkelvieva, T., & Ismailova, E. (2022). In vitro and in vivo screening of Beauveria bassiana strains for endophytic and insecticide activity. IV International Agricultural, Biological & Life Science Conference, Turkey.

Fiana, Y., Nurbani, N., & Danial, D. (2015). Study on the effectiveness of biological agent Beauveria bassiana and pod sleeving for controlling cocoa pod borer pest in east Kalimantan. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, 1(5), Article 5. /m010545

Gerding, M., France, A., Sepulveda, M., & Campos, J. (2007). Use of chitin to improve a Beauveria bassiana alginate-pellet formulation. Biocontrol Science and Technology, 17, 105–110. 1080/09583150600937717

Gul, H., Saeed, S., & Khan, F. Z. A. (2014). Entomopathogenic fungi as effective insect pest management tactic: A review. Applied Sciences and Business Economics, 1, 10–18

Harith-Fadzilah, N., Abd Ghani, I., & Hassan, M. (2021). Omics-based approach in characterising mechanisms of entomopathogenic fungi pathogenicity: A case example of Beauveria bassiana. Journal of King Saud University - Science, 33(2), 101332. /j.jksus.2020.101332

Herlinda, S., Utama, M. D., Pujiastuti, Y., & Suwandi, D. (2006). Kerapatan dan viabilitas spora Beauveria bassiana (Bals.) akibat subkultur dan pengayaan media, serta virulensinya terhadap larva Plutella xylostella (Linn.). Jurnal Hama dan Penyakit Tumbuhan Tropika, 6(2), 70–78. /j.hptt.2670-78

Indriyanti, D. R., Bintari, S. H., Setiati, N., & Alfiyan, J. M. Z. (2021). The density and viability of Metarhizium anisopliae conidia on several growth media. Biosaintifika: Journal of Biology & Biology Education, 13(2), Article 2.

Jaber, L. R., & Enkerli, J. (2017). Fungal entomopathogens as endophytes: Can they promote plant growth? Biocontrol Science and Technology, 27(1), 28–41. 1080/09583157.2016.1243227

Kidanu, S. (2020). Research and application of entomopathogenic fungi as pest management option: A review. Journal of Environment and Earth Science, 10(3), 31

Li, Z., Chambi, C., Du, T., Huang, C., Wang, F., Zhang, G., Li, C., & Juma Kayeke, M. (2019). Effects of water immersion and soil moisture content on larval and pupal survival of Bactrocera minax (Diptera: Tephritidae). Insects, 10(5), 138. insects10050138

Litwin, A., Nowak, M., & Różalska, S. (2020). Entomopathogenic fungi: Unconventional applications. Reviews in Environmental Science and BioTechnology, 19(1), 23–42. https://doi. org/10.1007/s11157-020-09525-1

Mustafa, U., & Kaur, G. (2009). Effects of carbon and nitrogen sources and ratio on the germination, growth and sporulation characteristics of Metarhizium anisopliae and Beauveria bassiana. African Journal of Agricultural Research, 3, 922–930

Nurmayulis, Hastuti, D., Eris, F. R., & Mujahidah. (2021). The symptomp rate of cocoa pod borer (Conopomorpha cramerella) due to the combination of several concentrations of Neem (Azadiracta indica) solution given the biosurfactant of diethanolamide olein palm and different fruit sizes. IOP Conference Series: Earth and Environmental Science, 715(1), 012048. /1/012048

Oliveira, D. G. P., Pauli, G., Mascarin, G. M., & Delalibera, I. (2015). A protocol for determination of conidial viability of the fungal entomopathogens Beauveria bassiana and Metarhizium anisopliae from commercial products. Journal of Microbiological Methods, 119, 44–52. 2015.09.021

Pelizza, S. A., Elíades, L. A., Saparrat, M. C. N., Cabello, M. N., Scorsetti, A. C., & Lange, C. E. (2012). Screening of Argentine native fungal strains for biocontrol of the grasshopper Tropidacris collaris: Relationship between fungal pathogenicity and chitinolytic enzyme activity. World Journal of Microbiology and Biotechnology, 28(4), 1359–1366. https://doi. org/10.1007/s11274-011-0935-8

Rahayu, R., & Umrah, U. (2012). Uji kemampuan formula Beauveria bassiana Balsamo. bentuk sediaan tablet untuk mengendalikan penggerek buah kakao Conopomorpha cramerella Snellen. Biocelebes, 6(1), Article 1. https://bestjournal.

Rahmawati, D., Wagiman, F. X., Harjaka, T., & Putra, N. S. (2017). Detection of cocoa pod borer infestation using sex pheromone trap and its control by pod wrapping. Jurnal Perlindungan Tanaman Indonesia, 21(1), Article 1. https://doi. org/10.22146/jpti.22659

Rohman, F. L., Saputro, T. B., & Prayogo, Y. (2017). Pengaruh penambahan senyawa berbasis kitin terhadap pertumbuhan cendawan entomo-patogen Beauveria bassiana. Jurnal Sains dan Seni ITS, 6(2), Article 2. 10.12962/j23373520.v6i2.23827

Roswanjaya, Y. P., Saryanah, N. A., Nawfetrias, W., Rosdayanti, H., & Putri, A. L. (2021). Selection of culture medium and incubation time for growth and production of beauvericin by local Beauveria bassiana. Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agricultural and Development, 59–65. absr.k.210609.011

Safavi, S. A., Shah, F. A., Pakdel, A. K., Reza Rasoulian, G., Bandani, A. R., & Butt, T. M. (2007). Effect of nutrition on growth and virulence of the entomopathogenic fungus Beauveria bassiana. FEMS Microbiology Letters, 270(1), 116–123. 1111/j.1574-6968.2007.00666.x

Saputro, T. B., Prayogo, Y., Rohman, F. L., & Alami, N. H. (2019). The virulence improvement of Beauveria bassiana in infecting Cylas formicarius modulated by various chitin based compounds. Biodiversitas Journal of Biological Diversity, 20(9), Article 9. 13057/biodiv/d200909

Senthilraja, G., Anand, T., Durairaj, C., Raguchander, T., & Samiyappan, R. (2010). Chitin-based bioformulation of Beauveria bassiana and Pseudomonas fluorescens for improved control of leafminer and collar rot in groundnut. Crop Protection, 29(9), 1003–1010.

Vega, F., Jackson, M., Mercadier, G., & Poprawski, T. (2003). The impact of nutrition on spore yields for various fungal entomopathogens in liquid culture. World Journal of Microbiology and Biotechnology, 19, 363–368. 1023/A:1023924304456

Vidhate, R. P., Dawkar, V. V., Punekar, S. A., & Giri, A. P. (2023). Genomic determinants of entomopathogenic fungi and their involvement in pathogenesis. Microbial Ecology, 85(1), 49–60.

Wang, H., Peng, H., Li, W., Cheng, P., & Gong, M. (2021). The toxins of Beauveria bassiana and the strategies to improve their virulence to insects. Frontiers in Microbiology, 12.

Włóka, E., Boguś, M. I., Wrońska, A. K., Drozdowski, M., Kaczmarek, A., Sobich, J., & Gołębiowski, M. (2022). Insect cuticular compounds affect Conidiobolus coronatus (Entomopthorales) sporulation and the activity of enzymes involved in fungal infection. Scientific Reports, 12(1), Article 1.








How to Cite

Nurhangga, E., Nawfetrias, W., Djamas, N., & Jufri, A. (2024). The effect of chitin on the effectiveness of Beauveria bassiana formulation product to control cocoa pod borer in vitro. Menara Perkebunan, 92(1).