The extract of Cavendish banana (Musa acuminata) peel as antioxidant and anti-aging agents in model yeast Schizosaccharomyces pombe


  • Theresia Yolanda Avelina Departemen Biologi, FMIPA, IPB University
  • Rika Indri Astuti Departemen Biologi, FMIPA, IPB University
  • Wulan Tri Wahyuni Departemen Kimia, FMIPA, IPB University



oxidative stress, chronological life span, H2O2, maceration, model organism


Banana peel is one of the wastes that can be explored for various applications. Cavendish is among the most highly consumed bananas in the world, which implies a high amount of banana peel waste. Our study evaluated the potential of Cavendish banana peels as the source of antioxidant and anti-aging agents. Antioxidant was extracted using three different solvents, i.e., water, ethyl acetate, and methanol. Each extract was then assayed for antioxidant activity in vitro using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant and anti-aging activity of the extracts were further evaluated at the cellular level using the yeast Schizosaccharomyces pombe as a model. Our data indicated that the methanol extract of banana peel showed the highest antioxidant activity with an IC50 value of 253.8 µg mL-1 compared to ethyl acetate and water, yet it was considered weak. Interestingly, the methanol extract (600 ppm) showed antioxidant and anti-aging activities at cellular levels in yeast S. pombe. Therefore, this research reveals the potential for developing banana peel methanol extract as a component of pharmaceutical or cosmetic products with antioxidant and anti-aging properties.


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Ariybah, Z. Q., Astuti, R. I., & Listiyowati, S. (2021). Cell longevity of yeast Saccharomyces cerevisiae by clove bud extract treatment may occur in sirtuin-independent pathway. OnLine Journal of Biological Sciences Original Research Paper, 21(3). 0.327

Astuti, R.I., Listyowati, S., & Wahyuni, W. T. (2019). Life span extension of model yeast Saccharomyces cerevisiae upon ethanol derived-clover bud extract treatment. IOP Conference Series: Earth and Environmental Science, 299(1).

Astuti, R.I., Nasuno, R., & Takagi, H. (2018). Nitric oxide signalling in yeast. Advances in Microbial Physiology, 72(45), 1–38. https://

Astuti, Rika Indri, Prastya, M. E., Batubara, I., Budiarti, E., & Ilmiyawati, A. (2021). Antiaging and antioxidant bioactivities of asteraceae plant fractions on the cellular functions of the yeast Schizosaccharomyces pombe. Advances in Pharmacological and Pharmaceutical Sciences, 2021, 2119634.

Batubara, I., Astuti, R. I., Prastya, M. E., Ilmiawati, A., Maeda, M., Suzuki, M., Hamamoto, A., & Takemori, H. (2020). The antiaging effect of active fractions and Ent-11α-Hydroxy-15-Oxo-Kaur-16-En-19-Oic acid isolated from Adenostemma lavenia (L.) O. Kuntze at the Cellular Level. Antioxidants, 9(8), 719.

Falcomer, A. L., Riquette, R. F. R., De Lima, B. R., Ginani, V. C., & Zandonadi, R. P. (2019). Health benefits of green banana consumption: a systematic review. Nutrients, 11(6).

Guo, C. Y., Sun, L., Chen, X. P., & Zhang, D. S. (2013). Oxidative stress, mitochondrial damage and neurodegenerative diseases. Neural Regeneration Research, 8(21), 2003–2014. 4.2013.21.009

Heriani, F. A., Sari, S. P., & Oktasari, A. (2021). Antioxidant activity of uli banana peel extract (Musa x Paradisiaca L. AAB). Stannum : Jurnal Sains Dan Terapan Kimia, 3(2), 64–68.

Ismail, N. Z., Arsad, H., Samian, M. R., & Hamdan, M. R. (2017). Determination of phenolic and flavonoid contents, antioxidant activities and GC-MS analysis of Clinacanthus nutans (Acanthaceae) in different locations. Agrivita, 39(3), 335–344. agrivita.v39i3.1076

Jun, M., Fu, H. Y., Hong, J., Wan, X., Yang, C. S., & Ho, C. T. (2003). Comparison of antioxidant activities of isoflavones from kudzu root (Pueraria lobata Ohwi). Journal of Food Science, 68(6), 2117–2122. 10.1111/J.1365-2621.2003.TB 07029.X

Kefayati, Z., Motamed, S. M., Shojaii, A., Noori, M., & Ghods, R. (2017). Antioxidant activity and phenolic and flavonoid contents of the extract and subfractions of Euphorbia splendida Mobayen. Pharmacognosy Research, 9(4), 362. /PR.PR_12 _17

Lesmana, D., Andrianto, D., & Astuti, R. I. (2021). Antiaging properties of the ethanol fractions of clove (Syzygium aromaticum L.) bud and leaf at the cellular levels: Study in yeast schizosaccharomyces pombe. Scientia Pharmaceutica, 89(4). /scipharm89040045

Luh, N., Wahyuni, P. O., Noer, I., Teguh, D., & Trisnanto, B. (2022). Sikap konsumen dalam pembelian buah pisang cavendish di pasar modern kota Bandar Lampung. Journal of Food System and Agribusiness, 6(2), 201–207. 455

Nurcholis, W., Sya’bani Putri, D. N., Husnawati, H., Aisyah, S. I., & Priosoeryanto, B. P. (2021). Total flavonoid content and antioxidant activity of ethanol and ethyl acetate extracts from accessions of Amomum compactum fruits. Annals of Agricultural Sciences, 66(1), 58–62. 04.001

Pan, Y., Schroeder, E. A., Ocampo, A., Barrientos, A., & Shadel, G. S. (2011). Regulation of yeast chronological life span by TORC1 via adaptive mitochondrial ROS signaling. Cell Metabolism, 13(6), 668–678. 10.1016/j.cmet.2011.03.018

Parvez, G. M., Tonu, J. F., Ara, R., Joarder, Md. Y. A., Milon, M. M. M., Sarker, R. K., Naznin, Most. A., Hossain, Md. S., Sultana, R., Parvin, S., & Kader, Md. A. (2023). Phytochemical and antioxidant comparison of different varieties of banana. Journal of Pharmacognosy and Phytochemistry, 12(1), 194–199. 3.v12.i1c.14574

Pintać, D., Majkić, T., Torović, L., Orčić, D., Beara, I., Simin, N., Mimica–Dukić, N., & Lesjak, M. (2018). Solvent selection for efficient extraction of bioactive compounds from grape pomace. Industrial Crops and Products, 111, 379–390. .2017.10.038

Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., & Bitto, A. (2017). Oxidative stress: harms and benefits for human health. Oxidative Medicine and Cellular Longevity, 2017, 1–13. 6763

Pudjas, N. T. G., Mubarik, N. R., Astuti, R. I., & Sudirman, L. I. (2022). Antioxidant activity of endophytic bacteria derived from hoya multiflora blume plant and their cellular activities on Schizosaccharomyces pombe. HAYATI Journal of Biosciences, 29(2), 214–221. 1

Putu, N., Astiti, A., & Yulihastuti, D. A. (2017). Determination of flavonoid, tannin and vitamin c content from methanol extract wrapping stone banana (Musa brachycarpa), ketip banana (Musa Paradisiaca Forma Typiaca) and kepok banana (Musa acuminata). Journal of Advances in Tropical Biodiversity and Environmental Sciences, 1(2), 33.

Rahal, A., Kumar, A., Singh, V., Yadav, B., Tiwari, R., Chakraborty, S., & Dhama, K. (2014). Oxidative stress, prooxidants, and antioxidants: The interplay. BioMed Research International, 2014. 2014/761264

Roux, A. E., Chartrand, P., Ferbeyre, G., & Rokeach, L. A. (2010). Fission yeast and other yeasts as emergent models to unravel cellular aging in eukaryotes. Journals of Gerontology - Series A Biological Sciences and Medical Sciences, 65(1), 1–8. 152

Sarima, Astuti, R. I., & Meryandini, A. (2019). Modulation of aging in yeast Saccharomyces cerevisiae by roselle petal extract (Hibiscus sabdariffa L.). American Journal of Biochemistry and Biotechnology, 15(1), 23–32.

Shalaby, E. A., & Shanab, S. M. M. (2013). Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. In Indian Journal of Geo-Marine Sciences (Vol. 42, Issue 5).

Smith, M. G., & Snyder, M. (2006). Yeast as a model for human disease. Current Protocols in Human Genetics, 48(1), 1–8. 10.1002/0471142905

Syaefudin, Wahyuni, W. T., Artika, I. M., & Sulistiyani. (2014). Antioxidant activity of flavonoid from Guazuma ulmifolia la ink. leaves and apoptosis induction in yeast cells. Journal 305.310

Tan, B. L., Norhaizan, M. E., Liew, W. P. P., & Rahman, H. S. (2018). Antioxidant and oxidative stress: A mutual interplay in age-related diseases. Frontiers in Pharmacology, 9(1162), 1–28. 2018.01162

Thavamoney, N., Sivanadian, L., Tee, L. H., Khoo, H. E., Prasad, K. N., & Kong, K. W. (2018). Extraction and recovery of phytochemical components and antioxidative properties in fruit parts of Dacryodes rostrata influenced by different solvents. Journal of Food Science and Technology, 55(7), 2523. 10.1007/S13197-018-3170-6

Trostchansky, A., Xu, S., Demicheli, V., Rahman, H. S., Tan, B. L., Norhaizan, M. E., & Liew, W.-P.-P. (2018). Antioxidant and oxidative stress: a mutual interplay in age-related diseases. Frontiers in Pharmacology, 9(1162), 1–28.

Truong, D. H., Ta, N. T. A., Pham, T. V., Huynh, T. D., Do, Q. T. G., Dinh, N. C. G., Dang, C. D., Nguyen, T. K. C., & Bui, A. V. (2021). Effects of solvent—solvent fractionation on the total terpenoid content and in vitro anti‐inflammatory activity of Serevenia buxifolia bark extract. Food Science & Nutrition, 9(3), 1720.

Tungmunnithum, D., Drouet, S., & Hano, C. (2022). Flavonoids from sacred lotus stamen extract slows chronological aging in yeast model by reducing oxidative stress and maintaining cellular metabolism. Cells, 11(4), 599. https://

Utami, L. A., Wahyuni, W. T., Mubarik, N. R., & Astuti, R. I. (2023). Endophytic bacteria of clove (Syzygium aromaticum L.) leaves produce metabolites with antioxidant and anti-aging properties. Journal of Applied Pharmaceutical Science, 13(7), 241–250.

Wa¸troba, M., & Szukiewicz, D. (2016). The role of sirtuins in aging and age-related diseases. Advances in Medical Sciences, 61(1), 52–62.

Wierman, M. B., & Smith, J. S. (2014). Yeast sirtuins and the regulation of aging. FEMS Yeast Research, 14(1), 73–88. 10.1111/1567-1364.12115

Yusuf, S. M., Astuti, R. I., Batubara, I., & Chavasiri, W. (2021). Anti-aging activity of Xylocarpus granatum phytoextracts and Xyloccensins K compound. Indonesian Journal of Pharmacy, 32(3), 365–375. 1430








How to Cite

Avelina, T. Y., Astuti, R. I., & Wahyuni, W. T. (2024). The extract of Cavendish banana (Musa acuminata) peel as antioxidant and anti-aging agents in model yeast Schizosaccharomyces pombe. Menara Perkebunan, 92(1).