Pengaruh kitosan terhadap peningkatan level ekspresi WRKY17 dan WRKY53 tanaman Capsicum annuum cv. Laba pada kondisi kekeringan
DOI:
https://doi.org/10.22302/iribb.jur.mp.v88i2.380Keywords:
red chili cv. Laba, gene expression, growth performanceAbstract
Chitosan is known as the natural plant growth biostimulant and defense elicitor involving WRKY transcription factors in response to environmental stresses. However, either plant’s growth or defense responses against stress are different among the cultivars. In the previous study, chitosan and drought treatment combination on red chili plant cv. Lado resulted in the impeded growth performance followed by the down-regulated expression level of WRKY17. Hence, this study aimed to investigate the response of red chili plant from the different cultivar towards the combination of chitosan and drought treatments. At the onset of the generative phase, plants were subjected to the drought treatment with and without 1 mg mL-1 of chitosan application. Observation of plant growth performance was carried out by measuring plant height, the number of leaves, the conversion percentage of flowers developed to fruits, and the number of harvested fruits, while the molecular parameter was indicated by the analyses of WRKY17 and WRKY53 expression levels. In line with the previous study using cv. Lado, the combination of 1 mg mL-1 of chitosan and drought treatment significantly reduced the plant productivity observed in the number of fruits, followed by the plant height and the number of leaves. However, the expression level of WRKY17 and WRKY53 experienced 10 and 22-fold higher than controls, respectively. This is different from the previous study which showed the reduction of WRKY17 expression level. According to the study, it can be implied that the combination of chitosan and drought treatments on red chili plant cv. Laba could reduce plant growth performance, but increased genetics indicator towards plant’s defense system against stress indicated by the escalation of WRKY17 and WRKY53 expression level. Furthermore, the individual chitosan application are potential to increase the productivity of red chili plant cv. Laba.
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Anjum, S. A., Xie, X., Wang, L., Saleem, M.F., Man, C., and Lei, W. 2011. Morphological, physiological, and biochemical responsses of plants to drought stress. African Journal of Agricultural Research, 6: 2016-2032.
Aziz, M.A., Esyanti, R.E., Meitha, K., Dwivany, F.M., and Chotimah, H.H., 2020. Chitosan suppresses the expression level of WRKY17 on red chili (Capsicum annuum) plant under drought stress. Indonesian Journal of Biotechnology. 2020.
Balitbang. (2016, Juli 11). Pengendalian Penyakit Antraknose pada Tanaman Cabai. Diakses pada tanggal 21 Agustus 2017 dari Badan Litbang Pertanian: http://www.litbang.pertanian.go.id/berita/one/2630/
Bistgani, Z. E., Siadat, S. A., Bakhshandeh, A., Pirbalouti, A. G., and Hashemi M. 2017. Interactive effects of drought stress and chitosan application on physiological characteristics and essential oil yield of Thymus daenensis Celak. The Crop journals, 407-415.
Cai, R., Dai, W., Zhang, C., Wang, Y., Wu, M., Zhao, Y., Ma, Q., Xiang, Y., and Cheng, B., 2017. The Maize WRKY Transcription Factor ZmWRKY17 Negatively Regulates Salt Stress Tolerance in Transgenic Arabidopsis plants. Planta. 2017.
Chookhongkha, N., Miyagawa, S., Jirakiattikul, Y., and Photchanchai, S. 2012. Chili growth and seed productivity as affected by chitosan. International Conference on Agriculture Technology and Food Sciences (ICATFS), Nov. 17-18 2012.
Ding, Z. J., Yan, J. Y., Li, G. X., Wu, Z. C., Zhang, S. Q., and Zheng, S. J. 2014. WRKY41 controls arabodopsis seed dormancy via direct regulation of ABI3 transcript levels not downstream of ABA. The Plant Journal, 79: 810-823.
Dorji, K., Behboudian, M.H., and Zegda-dominguez, J.A. 2005. Water relations, growth, yield, and fruit quality of hot pepper under deficit irrigation and partial rootzone drying. Scientia Horticulturae, 104: 137-149.
Dzung, Nguyen Anh, Khanh, Vo Thi P., and Dzung, Tran Trung. 2011. Research on impact of chitosan oligomers on biophysical characteristic, growth, development, and drought resistance of coffee. Carbohydrate Polymers, 84: 751-755.
El-Tanahy, A.M.M., Mahmoud, A.R., Mona, M. Abde-Mouty, and Ali, A. H. 2012. Effect of chitosan doses and nitrogen sources on the growth, yield and seed quality of cowpea. Australian Journal of Crop Science, 6: 115-121.
Esyanti RR, Dwivany FM, Mahani S, Nugrahapraja H, Meitha K. 2019. Foliar application of chitosan enhances growth and modulates expression of defense genes in chili pepper (Capsicum annuum L.). Aust J Crop Sci. 13(1):55–60. doi:10.21475/ajcs.19.13.01.p1169.
Golldack, D., Li, C., Mohan, H., and Probst, N. 2014. Tolerance to drought and salt stress in plants: unrevealing the signal networks. Frontiers Plant Science, 5: 1-10.
Iriti, Marcello, Picchi, V., Possomi, M., Gomarasca, S., Ludwig, N., Gargano, M., and Faoro, F. 2009. Chitosan antitranspirant activity is due to absisic acid-dependent stomatal closure. Environmental and Experimental Botany, 66: 493-500.
Jang, E., Gu, E., Hwang, B., Lee, C. and Kim, J. 2012. Chitosan stimulates calcium uptake and enhances the capability of chinese cabbage plant to resist soft rpt disease caused by Pectocacterium carotovorum spp. Carotovorum. Korean Journal of Horticultural Science and Technology, 30: 137-143.
Jiang, Chun-Hao, Huang, Zi-Yang, Xie, P., Gu, C., Li, K., Wang, Da-Chen, Yu, Yi-Yang, Fan, Zhi-Hang, Wang, Chun-Juan, Wang, Yun-Peng, Guo, Ya-Hui, and Guo, Jian-Hua. 2015. Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium bacillus cereus ar156-indeced systemic resistance to Pseudomonas syringae pv. Tomato DC3000 in Arabidopsis. Journal of Experimental Botany, 1-18.
Journot-Catalino, N., Somssich, Imre E., Roby, D., and Kroj, T. 2006. The transcription factors WRKY11 and WRKY17 act as negative regulator of basal resistence in Arabidobsis thaliana. The Plant Cell, 18: 3289-3302.
Karimi, S., Abbaspour, H., Sinak, J. M., and Makarian, H., 2012. Evaluation of drought stress and foliar chitosan on biochemical characterices of castor bean (Ricinus communis L.). Research Journal of Biological Sciences. 7: 117-122.
Kementerian Pertanian. 2015. Statistik Produksi Hortikultura 2014. Jakarta : Direktorat Jendral Hortikultura, Kementarian Pertanian.
Kementerian Pertanian. 2016. Outlook Komoditas Pertanian Sub Sektor Hortikultura : Cabai. Jakarta : Pusat Data dan Sistem Informasi Pertanian, kementerian Pertanian.
Khan, Abdul Latif, Shin, Jae-Ho, Jung, Hee-Young, and Lee, In-Jung. 2014. Regulation of capsaicin synthesis in Capsicum annum L. by Penicillium resedanum LK6 during drought condition. Scientia Horticulturae, 175: 167-173.
Livak, K.J., and Schmittgen, T. D. 2001. Analisys of relative gene expression data using real time quantitative pcr and the 2-ΔΔCT method. Methods, 25 : 402-408.
Malekpoor, F., Pirbalouti, A. G., and Salimi, A., 2016. Effect of Foliar Application of Chitosan on Morphological and Physiological Charactristics of Basil Under Reduced Irrigation. Research on Crops, 17: 354-359.
Malerba, Massimo, and Cerana, Raffaella. 2016. Chitosan effects on plant system. International Journal of Molecular science, 17: 996.
Maser, Pascal, Leonhardt, N., and Schroeder, Julian L. 2018. The clickable guard cell: electronically linked model of guard cell signal transduction pathways. Schroader Laboratory, UCSD, diakses pada Desember 2018 : http://labs.biology.ucsd.edu/schroeder/clickablegc.html.
Mondal, M.M.A., Malek, M.A., Puteh, A.B., Ismail, M.R., Ashrafuzzman, M., and Naher, L. 2012. Effect of foliar application of chitosan on growth and yield in okra. Australian Journal of Crop Science, 6: 918-921.
Niu, Can-Fang, Wei, W., Zhou, Oi-Yun, Tian, Ai-Guo, Hao, Yu-Jun, Zhang, Wan-Ke, Ma, Biao, Lin, Qing, Zhang, Zheng-Bin, Zhang, Jin-Song, and Chen, Shou-Yi. 2012. Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic arabidopsis plants. Plant, Cell and Environment, 1-15.
Ohta K, Morishita S, Suda K, Kobayashi N, Hosoki T. 2004. Effects of chitosan soil mixture treatment in the seedling stage on the growth and flowering of several ornament plants. Journal of the Japanese Society for Horticultural Science, 73: 66-68.
Phimchan, P., Techawongstein, S., Chanthai, S., and Bosland, Paul W. 2012. Impact of drought stress on the accumulation of capsaicinoids in capsicum cultivars with different initial capsaicinoid levels. HortScience, 47: 1204-1209.
Phukan, Ujjal, J., Jeena, G. S., and Shukla, R. K. 2016. WRKY transcription factor: molecular regulation and stress responsses in plants. Frontiers In Plant Science, 7: 1-14.
Pichyangkura, R., and Chadchawan, S. 2015. Biostimulant activity of chitosan in horticulture. Scientia Horticulturae, 196: 49-65.
Salachna, Piotr, and Zawadzinska. 2014. Effect of chitosan on plant growth, flowering, and corms yield of potted freesia. Journal of Ecological Engineering, 15: 97-102.
Sumarni, N., and Muharam, A., 2005. Budidaya Tanaman Cabai Merah. Bandung: Balai Penelitian Tanaman Sayur.
Sun, Yiding, and Yu, Diqiu. 2015. Activated expression of AtWRKY53 negatively regulates drought tolerance by mediating stomatal movement. Plant Cell Report, 2015.
Sung, Yu, Chang, Yu-Yin, and Ting, Ni-Lun. 2005. Capsaicin biosynthesis in water-stressed hot pepper fruits. Botanical Bulletin of Academia Sinica, 46: 35-42.
Yan, H., Jia, H., Chen, X., Hao, L., An, Hailong, and Guo X. 2014. The cotton WRKY transcription factor GhWRKY17 functions in drought and salt in transgenic Nicotiana benthamiana through ABA signaling and the modulation of reactive oxygen species production. Plant and Cell Physiology. 55: 2060-2076.
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