Gibberellic acid (GA) role in acetyl-coA carboxylase enzyme regulation and in improving oil palm yield

Authors

DOI:

https://doi.org/10.22302/iribb.jur.mp.v91i2.533

Keywords:

ACCs, WRI1, TCP4, phosphorylation, fatty acids

Abstract

Seaweed specifically, Sargassum sp. is known to contains a boosting hormone growth that has been promoted plant growth and yield due to the containing of auxin, gibberelic acid (GA) and cytokinine, and also some amino acids especially glutamic acid. Those composition could be used as an booster of palm oil production which related to acetyl co-A carboxylase activity (ACC). ACC is the rate determination step in fatty acid accumulation, and becomes active through dephosphorylation of some serine residues that induced by magnesium and glutamate. Moreover, ACC was regulated by AtWRI1 and AtWRI1-TCP4 interaction, a mechanism that allow fine-tuning of the oil biosynthetic pathway. In this research we conducted gene expression experiments, and molecular docking analyses to study the possible mechanism of seaweed composition  stimulating oil  accumulation  in the    oil palm. Further  analysis  was  conducted  to  ensure whether the interaction between TCP4 and candidate inhibitors were able to phosphorylate TCP4 and decrease its activity. GA application resulted in the increase of oil accumulation in 1 month after application, although in the second month the oil accumulation showed decreasing. Increase of oil accumulation in the first month in line with the increase of the expression of ACC in 3rd and 5th weeks. Meanwhile, TCP4 showed decrease expression that resulted in the increase of the WRI1 in 5th week. From this result, it was indicated that GA application could block the TCP4, so it could not interact with WRI1, resulted in the expression of WRI1 and ACC. This interaction stimulates the oil accumulation in oil palm.

 

Downloads

Download data is not yet available.

Author Biography

Irma Kresnawaty, Indonesian Oil Palm Research Institute Bogor Unit

Bioindustry Reasearch Group

Young Researcher

References

Ali, O., Ramsubhag, A., & Jayaraman, J. (2021). Biostimulant properties of seaweed extracts in plants: Implications towards sustainable crop production. Plants, 10(3), 531. https://doi.org/ 10.3390/plants10030531

Chandel, V., Raj, S., Rathi, B., & Kumar, D. (2020). In silico identification of potent COVID-19 main protease inhibitors from FDA approved antiviral compounds and active phytochemicals through molecular docking: a drug repurposing approach.

Cocozza, C., Parente, A., Zaccone, C., Mininni, C., Santamaria, P. & Miano, T. (2011). Comparative management of offshore posidonia residues: composting vs. energy recovery. Waste Management (Oxford), 31, 78–84. https://doi.org/10.1016/j.wasman. 2010.08.016

Gaussin, V., Hue, L., Stalmans, W. & Bollen, M. (1996). Activation of hepatic acetyl-CoA carboxylase by glutamate and Mg2+ is mediated by protein phosphatase-2A. Biochemical Journal, 316(1), 217-224. https://doi.org/10.1042 /bj3160217

Hardie G.D & Carling D. (1997). The AMP-activated protein kinase: Fuel gauge of the mammalian cell. European Journal of Biochemistry, 246, 259 –273. https: //doi.org/10.1111/j.1432-1033.1997. 00259.x

Haas, J., Roth, S., Arnold, K., Kiefer, F., Schmidt, T., Bordoli, L., & Schwede, T. (2013). The Protein Model Portal—a comprehensive resource for protein structure and model information. Database, 2013. https://doi.org/ 10.1093/database /bat031

Kong, Q., Singh, S. K., Mantyla, J. J., Pattanaik, S., Guo, L., Yuan, L., & Ma, W. (2020). Teosinte Branched1/Cycloidea/Proliferating Cell Factor4 Interacts with Wrinkled1 to mediate seed oil biosynthesis. Plant Physiology, 184(2), 658-665. https://doi.org/10.1104/ pp.20.00547

Kong, Q., Yang, Y., Guo, L., Yuan, L., & Ma, W. (2020). Molecular basis of plant oil biosynthesis: Insights gained from studying the WRINKLED1 transcription factor. Frontiers In Plant Science, 11, 24. https://doi.org/10.3389/fpls.2020 .00024

Kowluru, A H Q Chen, L M. Modrick & C Stefanelli. (2001). Activation of Acetyl-CoA Carboxylase by a Glutamate and Magnesium-Sensitive Protein Phosphatase in the Islet b-Cell. Diabetes, 50. https://doi.org/10.2337/ diabetes.50.7.1580

Kresnawaty, I. Priyono, A. Budiani, D.A Sari, S. Hudiyono & D.Santoso. (2023). Seaweed extract-based biotimulant increased oil yield of early productive oil palms. (unpublished)

Kubota, A., Ito, S., Shim, J. S., Johnson, R. S., Song, Y. H., Breton, G., & Imaizumi, T. (2017). TCP4-dependent induction of CONSTANS transcription requires GIGANTEA in photoperiodic flowering in Arabidopsis. PLoS Genetics, 13(6), e1006856. https://doi.org/10. 1371/journal.pgen.1006856

Liu, Y., Guan, X., Liu, S., Yang, M., Ren, J., Guo, M., & Zhang, Y. (2018). Genome-wide identification and analysis of TCP transcription factors involved in the formation of leafy head in Chinese cabbage. International Journal of Molecular Sciences, 19(3), 847. https://doi.org/ 10.3390/ijms19030847

Lu, Y., Yuan, M., Gao, X., Kang, T., Zhan, S., Wan, H., & Li, J. (2013). Identification and validation of reference genes for gene expression analysis using quantitative PCR in Spodoptera litura (Lepidoptera: Noctuidae). PloS One, 8(7), e68059. https://doi.org/10.3390/life12091379

Ohlrogge, J. B., Jaworski, J. G., & Post-Beittenmiller, D. (2018). De novo fatty acid biosynthesis. In Lipid Metabolism in Plants (pp.3-32). CRC Press. https://doi.org/10.1201/ 9781351074070-2

Gibberellic acid (GA) role in acetyl co-A carboxylase enzyme regulation and in improving oil palm yield….....(Kresnawaty et al.)

Perez, M., Guerringue, Y., Ranty, B., Pouzet, C., Jauneau, A., Robe, E., & Aldon, D. (2019). Specific TCP transcription factors interact with and stabilize PRR2 within different nuclear sub-domains. Plant Science, 287, 110-197. https://doi.org/10.1016/j.plantsci. 2019.110197

Pradhan, B., Bhuyan, P. P., Patra, S., Nayak, R., Behera, P. K., Behera, C., & Jena, M. (2022). Beneficial effects of seaweeds and seaweed-derived bioactive compounds: Current evidence and future prospective. Biocatalysis and Agricultural Biotechnology, 39, 102242. https://doi.org/10.1016/j.bcab.2021.102242

Putranto, R. A., Leclercq, J., & Montoro, P. (2015). Evaluation of eleven reference genes for Reverse Transcriptase Quantitative PCR of rubber tree under water. Menara Perkebunan, 83(2), 86–94 https://doi.org/10.22302/iribb. jur.mp.v83i2.5

Vavvas D, A Apazidis, A Saha, J Gamble, A Patel, BE Kemp, LA Witters, & NB Ruderman. (1997). Contraction-induced changes in acetyl CoA carboxylase and 59-AMP-activated kinase in skeletal muscle. Journal of Biological Chemistry, 272, 13255–13261. https://doi.org/10.1074/jbc. 272. 20.13255

Downloads

Submitted

06-03-2023

Accepted

13-07-2023

Published

25-10-2023

How to Cite

Kresnawaty, I., Santoso, D., Permatasari, G. W., & Hudiyono, S. (2023). Gibberellic acid (GA) role in acetyl-coA carboxylase enzyme regulation and in improving oil palm yield. Menara Perkebunan, 91(2), 130–139. https://doi.org/10.22302/iribb.jur.mp.v91i2.533

Most read articles by the same author(s)

<< < 1 2 3 4 5 > >>