Peningkatan kadar capsaicin tanaman Capsicum annuum cv. Lado pada kondisi kekeringan menggunakan kitosan

Muhammad Abdul AZIZ, Sri WAHYUNI, Fenny Martha DWIVANNY, Rizkita Rachmi ESYANTI

Abstract


Abstrak

Cabai merah (Capsicum annuum) merupakan jenis sayuran yang memiliki nilai ekonomi tinggi. Saat kekeringan, kemampuan bertahan hidup tanaman tersebut sering kali menurun, sehingga diperlukan upaya untuk meningkatkan ketahanannya. Kitosan dikenal sebagai elisitor ketahanan tanaman terhadap infeksi patogen melalui induksi sintesis metabolit sekunder senyawa golongan fenol seperti capsaicin. Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi kitosan 1 mg mL-1 terhadap peningkatan kadar capsaicin dan ekspresi gen PAL1 tanaman cabai merah cv. Lado pada kondisi kekeringan. Serial perlakuan terdiri dari kitosan (Chi), kombinasi kitosan dan kekeringan (Chi-D), kekeringan (D) dan kontrol (C) yang diaplikasikan saat tanaman memasuki fase generatif. Parameter yang diamati meliputi analisis ekspresi gen PAL1, aktivitas enzim PAL dan kadar capsaicin. Hasil penelitian menunjukkan bahwa aplikasi kitosan 1 mg mL-1 saat kekeringan menurunkan level ekspresi gen PAL1 0,61 kali dan aktivitas enzim PAL 0,94 kali dibanding kontrol. Sebaliknya, kadar capsaicin meningkat 2,46 kali dibandingkan kontrol, sehingga aplikasi kitosan 1 mg mL-1 saat kekeringan diduga dapat meningkatkan ketahanan tanaman tersebut terhadap infeksi patogen.


Keywords


cabai merah, ekspresi gen PAL1, metabolit sekunder

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References


Anjum SA, X Xie, L Wang, MF Saleem, C Man & W Lei (2011). Morphological, physiological, and biochemical responses of plants to drought stress. African Journal of Agricultural Research 6(9), 2016-2032.

Arora RS, NG Gill, C Chauhan & A Rana (2011). An overview about versatile molecule capsaicin. International Journal of Pharmaceutical Sciences and Drug Research 3, 280-286.

Aziz MA, RE Esyanti & FM Dwivanny (2020a). Pengaruh kitosan terhadap peningkatan level ekspresi WRKY17 dan WRKY53 tanaman Capsicum annuum cv. Laba pada kondisi kekeringan. Menara Perkebunan 88(2), 120-129.

Aziz MA, RE Esyanti, K Meitha, FM Dwivany & HH Chotimah (2020b). Chitosan suppresses the expression level of WRKY17 on red chili (Capsicum annuum) plant under drought stress. Indonesian Journal of Biotechnology, 25(1), 52-60.

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem, 72: 248- 254.

Dorji K, MH Behboudian & JA Zegda-dominguez (2005). Water relations, growth, yield, and fruit quality of hot pepper under deficit irrigation and partial rootzone drying. Scientia Horticulturae 104, 137-149.

Dzung NA, VTP Khanh & TT Dzung (2011). Research on impact of chitosan oligomers on biophysical characteristic, growth, develop- ment, and drought resistance of coffee. Carbohydrate Polymers 84, 751-755.

Esyanti RR, FM Dwivany, S Mahani, H Nugrahapraja & K Meitha (2019). Foliar application of chitosan enhances growth and modulates expression of defense genes in chili pepper (Capsicum annuum L.). Aust J Crop Sci 13(1), 55-60.

Goci E, E Haloci, K Vide & L Malaj (2013). Application and comparison of three different extraction methods of capsaicin from capsicum fruits. Albanian Journal of Pharmaceutical Sciences 1(1), 16-19.

Golldack D, C Li, H Mohan & N Probst (2014). Tolerance to drought and salt stress in plants: unrevealing the signal networks. Frontiers Plant Science 151(5), 1-10.

Gonzales-zamora A, E Sierra-campos, JG Luna-ortega, R Perez-morales, JC Ortiz & JL Garcia-hernandez (2013). Characterization of different capsicum varieties by evaluation of their capsaicinoids content by high performance liquid chromatography, determination ofpungency and effect of high temperature. Molecules 18, 13471-13486.

Ibrahim MH & HZE Jaafar (2013). Absisic acid induced changes in production of primary and secondary metabolites, photosynthetic capacity, antioxidant capability, antioxidant enzymes, and lipooxygenase inhibitory activity of Orthosiphon stamineus Benth. Molecules 18, 7957-7976.

Iriti M, V Picchi, M Possomi, S Gomarasca, N Ludwig, M Gargano & F Faoro (2009). Chitosan antitranspirant activity is due to absisic acid-dependent stomatal closure. Environmental and Experimental Botany 66, 493-500.

KEMENTAN (2016). Outlook Komoditas Pertanian Sub Sektor Hortikultura : Cabai. Jakarta: Pusat Data dan Sistem Informasi Pertanian, kementerian Pertanian.

Khan AL, JH Shin, HY Jung & IJ Lee (2014). Regulation of capsaicin synthesis in capsicum annum L. by Penicillium resedanum LK6 during drought condition. Scientia Horticulturae 175, 167-173.

Khan W, B Prithiviraj, Smith & L Donald (2003). Chitosan and chitin oligomers increase phenylalanine ammonia-lyase and tyrosine ammonia-lyase activities in soybean leaves. Journal of Plant Physiology 160, 859-863.

Kim DS & BK Hwang (2014). An important role of the pepper phenylalanine ammonia-lyase gen (PAL1) in salisilic acid-dependent signaling of the defence response to microbial pathogens. Journal of Experimental Botany 65(9), 2295-2306.

Kraikruan W, S Sangchote, & S Sukprakarn (2008). Effect of Capsaicin on Germination of Colletotrichum capsici Conidia. Kasetsart Journal (Natural Science) 42, 417-422.

Livak KJ & TD Schmittgen (2001). Analysis of relative gene expression data using real time quantitative pcr and the 2-ΔΔCT method. Methods 25, 402-408.

Malerba M & R Cerana (2016) Chitosan effects on plant system. International Journal of Molecular science 17, 996.

Mejia-teniente L, FD Duran-flores, AM Chapa-oliver, I Torres-pacheco, A Cruz-hernandez, M Gonzalez-chavira, M Ocampo-valazquez, V Rosalia & RG Guevara-gonzalez (2013). Oxydative and molecular responsses in Capsicum annuum L. after hydrogen peroxyde, salisilic acid and chitosan foliar applications. International Journal of Molecular Science 14, 10178-10196.

Mondal MMA, MA Malek, AB Puteh, MR Ismail, M Ashrafuzzman & L Naher (2012). Effect of foliar application of chitosan on growth and yield in okra. Australian Journal of Crop Science 6, 918-921.

Mukta JA, M Rahman, AA Sabir, DR Gupta, MZ Surovy, M Rahman & MT Islam (2017). Chitosan and plant probiotics application enhance growth and yield of strawberry. J Biocatal Agric Biotechnol 11, 9-18.

Phimchan P, S Techawongstein, S Chanthai & PW Bosland (2012) Impact of drought stress on the accumulation of capsaicinoids in capsicum cultivars with different initial capsaicinoid levels. HortScience 47, 1204-1209.

Pichyangkura R & S Chadchawan (2015). Biostimulant activity of chitosan in horticulture. Scientia Horticulturae 196, 49-65.

Sung Y, Y Chang & N Ting (2005). Capsaicin biosynthesis in water-stressed hot pepper fruits. Botanical Bulletin of Academia Sinica 46, 35-42.

Veloso J, C Prego, MM Varela, R Carballeira, A Bernal, F Merino & J Diaz (2013). Properties of capsaicinoids for the control of fungi and oomycetes pathogenic to pepper. Plant Biology 16, 177-185.

Wahyuni S, CA Yusup, DD Eris, SM Putra, AS Mulyani, Siswanto & Priyono (2019) Peningkatan hasil panen dan penekanan kejadian penyakit pada jagung manis (Zea mays var. Bonanza) dengan pemanfaatan biostimulan berbahan kitosan. Menara Perkebunan 87(2), 131-139.

Wahyuni S, R Selvina, R Fauziyah, HT Prakoso, Priyono & Siswanto (2020). Optimasi suhu dan waktu deasetilasi kitin berbasis selongsong maggot (Hermetia Illucens) menjadi kitosan. Jurnal Ilmu Pertanian Indonesia 25(3), 375-383.

Wasternack C (2013). Action of jasmonates in plants stress responses and development – applied aspect. Biotechnology Advances 32(1), 31-39.




DOI: http://dx.doi.org/10.22302/iribb.jur.mp.v89i2.423

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