Identification of volatile organic compound of oil palm plants infected with Ganoderma sp.
DOI:
https://doi.org/10.22302/iribb.jur.mp.v92i1.534Keywords:
benzaldehyde, GC-MS, Lignin, marker compounds, volatile compoundAbstract
The success of controlling basal stem rot (BSR) disease caused by Ganoderma sp. is mostly determined by the early detection of the fungi. Hence, when the signs of infection begin to appear, plants are generally difficult to rescue since the pathogen infection has spread widely to all parts of the plant. Therefore, early detection through the application of biosensors for Ganoderma sp. infection is absolutely necessary. This study aim was to identify biomarker compounds of Ganoderma sp. infection in oil palm plants with GC-MS for volatile compounds, and LC-MS for non-volatile compounds. The results showed that Ganoderma sp. mycelium produced pyrimidinamine compounds. Meanwhile in early infected nursery plants, were found benzo[h]quinoline, hexaoxa-7,9,11-trisilaheptad, tris-(trimethyl-silyl ester and methyl-tris(trimethyl-siloxy)-silane). Whereas in Bekri, Rejosari, and Adolina plantation, the compound methyl-tris(trimethyl-siloxy)-silane was also found in healthy mature plants. GC-MS test results showed that for early, moderate and severe plants produced several benzene derivative compounds such as ethylbenzene, xylene, and benzaldehyde. These compounds were assumed to be resulted from the breakdown of the lignin structure which build plant cell walls, and have potency to be used as marker compounds for early infected Ganoderma sp. detection. The result of the produced gas quantification concluded that in the produced oil palm plants released less CO2 compared to healthy plants. On the other hand, the NH3 produced was higher than the healthy plants. Meanwhile, two non-volatile compounds were found that they were only produced by infected trunk and root tissue, namely pseudobrucine and picrasidine.
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References
Ariffin, D., Idris, A. S., & Singh, G. (2000). Status of Ganoderma in oil palm. In Ganoderma diseases of perennial crops, 49-68. Wallingford UK: CABI
Badan Pusat Statistik. 2022. Statistik Kelapa Sawit 2022. Bogor: BPS.
Bennett, J. W., & Inamdar, A. A. (2015). Are some fungal volatile organic compounds (VOCs) mycotoxins. Toxins, 7(9), 3785-3804. https://doi. org/10.3390/toxins7093785
Blasioli, S., Biondi, E., Braschi, I., Mazzucchi, U., Bazzi, C., & Gessa, C. E. (2010). Electronic nose as an innovative tool for the diagnosis of grapevine crown gall. Analytica Chimica Acta, 672(1-2), 20-24. https://doi.org/10.1016/j. aca.2010.02.017
Chen, M., Xiao, D., Liu, W., Song, Y., Zou, B., Li, L., & Xie, Z. (2020). Intake of Ganoderma lucidum polysaccharides reverses the disturbed gut microbiota and metabolism in type 2 diabetic rats. International Journal of Biological Macromolecules, 155, 890-902. https://doi.org/ 10.1016/j.ijbiomac.2019.11.047
Currie, H. A., & Perry, C. C. (2009). Chemical evidence for intrinsic ‘Si’ within equisetum cell walls. Phytochemistry, 70(17), 2089-2095. http:// dx.doi.org/10.1016/j.phytochem.2009.07.03 9
De, B., & Bisset, N. G. (1991). Separation of Strychnos nux-vomica alkaloids by high-performance liquid chromatography. Journal of Chromatography A, 587(2), 318-320. https://doi .org/10.1016/0021-9673(91)85175-F
Hamada, S., Kontani, M., Hosono, H., Ono, H., Tanaka, T., Ooshima, T., & Abe, I. (1996). Peroxidase-catalyzed generation of catechin oligomers that inhibit glucosyltransferase from Streptococcus sobrinus. FEMS Microbiology Letters, 143(1), 35-40. https://doi.org/10.1016/03 78-1097(96)00273-X
Kataoka, M., Hirano, T., Kuroda, K., & Hayakawa, Y. (2005). Pyrimido [4, 5-d] pyrimidine-2, 4, 5, 7-(1 H, 3 H, 6 H, 8 H)-tetraone as a novel universal base. In Nucleic Acids Symposium Series (Vol. 49, No. 1, pp. 119-120). Oxford University Press. https://doi.org/10.1093/ nass/49.1.119
Kushalappa, A. C., Lui, L. H., Chen, C. R., & Lee, B. (2002). Volatile fingerprinting (SPME-GC-FID) to detect and discriminate diseases of potato tubers. Plant Disease, 86(2), 131-137. https://doi. org/10.1094/PDIS.2002.86.2.131
Kushairi, A., Ong-Abdullah, M., Nambiappan, B., Hishamuddin, E., Bidin, M. N. I. Z., Ghazali, R., & Parveez, G. K. A. (2019). Oil palm economic performance in Malaysia and R&D progress in 2018. Journal of Oil Palm Research, 31(2), 165-194. http://jopr.mpob.gov.my/wpcontent/uploads /2019/06/joprinpress2019-kushairi.pdf
Ky, C. L., Louarn, J., Dussert, S., Guyot, B., Hamon, S., & Noirot, M. (2001). Caffeine, trigonelline, chlorogenic acids and sucrose diversity in wild Coffea arabica L. and C. canephora P. accessions. Food Chemistry, 75(2), 223-230. https://doi. org/10.1016/S0308-8146(01)00204-7
Lee, Y. (2017). Cancer chemo preventive potential of procyanidin. Toxicological Research, 33, 273-282. https://doi.org/10.5487/TR.2017.33.4.273
LI, H. Y., Koike, K., & Ohmoto, T. (1993). New alkaloids, picrasidines W, X and Y, from Picrasma quassioides and X-ray crystallographic analysis of picrasidine Q. Chemical and Pharmaceutical Bulletin, 41(10), 1807-1811. https://doi.org/ 10.1248/cpb.41.1807
Nascimento, E., Da Silva, S. H., dos Reis Marques, E., Roberts, D. W., & Braga, G. U. (2010). Quantification of cyclobutane pyrimidine dimers induced by UVB radiation in conidia of the fungi Aspergillus fumigatus, Aspergillus nidulans, Metarhizium acridum and Metarhizium robertsii. Photochemistry and Photobiology, 86(6), 1259-1266. https://doi.org/10.1111/j.1751-1097.2010.00793.x
Nusaibah, S. A., Akmar, A. S. N., Idris, A. S., Sariah, M., & Pauzi, Z. M. (2016). Involvement of metabolites in early defense mechanism of oil palm (Elaeis guineensis Jacq.) against Ganoderma disease. Plant Physiology and Biochemistry, 109, 156-165. https://doi.org/10.10 16/j.plaphy.2016.09.014
Pan, L., Zhang, W., Zhu, N., Mao, S. and Tu, K. (2014). Early detection and classification of pathogenic fungal disease in post-harvest strawberry fruit by electronic nose and gas chromatography–mass spectrometry. Food Research International, 62: 162-168. https:// doi.org/10.1016/j.foodres.2014.02.020
Prats, E., Mur, L. A., Sanderson, R., & Carver, T. L. (2005). Nitric oxide contributes both to papilla‐based resistance and the hypersensitive response in barley attacked by Blumeria graminis f. sp. hordei. Molecular Plant Pathology, 6(1), 65-78. https://bsppjournals.onlinelibrary.wiley. com/doi/pdf/10.1111/j.131364-3703.2004.00266x
Rees, R. W., Flood, J., Hasan, Y., Potter, U. and Cooper, R. M. (2009). Basal stem rot of oil palm (Elaeis guineensis); Mode of root infection and lower stem invasion by Ganoderma boninense. Plant Pathology, 58: 982-989. https://doi.org/ 10.1111/j.1365-3059.2009.02100x
Seman, I. A., & Kamarudin, N. (2023). Standard operating procedures (SOP) guidelines for managing Ganoderma disease in oil palm. http://sawitsecure.mpob.gov.my/standard-operatin g-procedures-sop/
Spinelli, F., Costa, G., Rondelli, E., Busi, S., Vanneste, J. L., Rodriguez, E. M. T., Savioli, S. and Cristescu, S. M. (2011). Volatile compounds produced by Erwinia amylovora and their potential exploitation for bacterial identification. Acta Horticulturae, 896: 77-84. https://doi. org/10.17660/ActaHortic.2011.896.8
Triyana, K., Taukhid-Subekti, M., Aji, P., Hidayat, S.N. & Rohman, A. (2015). Development of electronic nose with low-cost dynamic headspace for classifying vegetable oils and animal Fats. Applied Mechanical Material, 771,50-54. https://doi.org/10.4028/www.scientific.net/AMM. 771.50
Wilson, A. D. (2018). Application of electronic-nose technologies and VOC-biomarkers for the noninvasive early diagnosis of gastrointestinal diseases. Sensors, 18(8), 2613. https://doi.org /10.3390/s18082613
Widada, J., Damayanti, E., Alhakim, M. R., Yuwono, T., & Mustofa, M. (2021). Two strains of airborne Nocardiopsis alba producing different volatile organic compounds (VOCs) as biofungicide for Ganoderma boninense. FEMS Microbiology Letters, 368 (20). https://doi.org/10.1093/femsle /fnab138
Yu, G., & Chong, K. (2018). Selected biomarkers from oil palm-Ganoderma infected tissues for detection of basal stem rot disease. WMSU Research Journal, 37(1), 1-13. https:// doi.org/10.1111/j.1365-3059.2009.02100.x
Zhang, H. N., He, J. H., Yuan, L., & Lin, Z. B. (2003). In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan- induced pancreatic islets damage. Life Sciences, 73(18), 2307-2319. https://doi.org/ 10.1016/S0024-3205(03)00594-0
Zhen, X. T., Chen, Y., Yu, Y. L., Shi, M. Z., Yan, T. C., Yue, Z. X., & Cao, J. (2021). In situ effervescence reaction-assisted mechanoche mical extraction of ganoderic acids from Ganoderma lucidum. Industrial Crops and Products, 168, 113577. https://doi.org/10.1016/j.indcrop.2021. 113577
Zhou, W., Zhu, X. X., Yin, A. L., Cai, B. C., Wang, H. D., Di, L., & Shan, J. J. (2014). Effect of various absorption enhancers based on tight junctions on the intestinal absorption of forsythoside A in Shuang-Huang-Lian, application to its antivirus activity. Pharmacognosy Magazine, 10(37), 9. https: //doi.org/10.4103%2F0973-1296.126651
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